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Updated: Feb 26, 2026

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices
Published on: November 25, 2013
Explant Methodology for Analyzing Neuroblast Migration
Kirsty J Dixon1, Alisa Turbic2, Ann M Turnley3
1Department of Transplant Surgery, Virginia Commonwealth University, Richmond, USA.
Abstract:
The subventricular zone (SVZ) in the mammalian forebrain contains stem/progenitor cells that migrate through the rostral migratory stream (RMS) to the olfactory bulb throughout adulthood. SVZ-derived explant cultures provide a convenient method to assess factors regulating the intermediary stage of neural stem/progenitor cell migration. Here, we describe the isolation of SVZ-derived RMS explants from the neonatal mouse brain, and the conditions required to culture and evaluate their migration.

