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The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications
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Recent Developments in Thiolated Polymeric Hydrogels for Tissue Engineering Applications.

Mani Gajendiran1, Jae-Sung Rhee2, Kyobum Kim1

  • 11 Division of Bioengineering, College of Life Sciences and Bioengineering, Incheon National University , Incheon, Korea.

Tissue Engineering. Part B, Reviews
|July 21, 2017
PubMed
Summary
This summary is machine-generated.

This review details strategies for creating thiolated polymers and hydrogels. These advanced materials show excellent biocompatibility and support cell growth, making them promising for tissue engineering applications.

Keywords:
disulfide bond formationhydrogel fabricationinterpenetrating polymer networkthiolated polymerstissue engineering

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Area of Science:

  • Polymer Chemistry
  • Biomaterials Science
  • Tissue Engineering

Background:

  • Thiolated polymers are crucial for developing advanced hydrogel matrices.
  • Recent strategies focus on efficient synthesis and fabrication of these materials.
  • Understanding thiolation mechanisms is key for tailoring polymer properties.

Purpose of the Study:

  • To review recent strategies in preparing thiolated polymers and fabricating hydrogel matrices.
  • To exemplify synthesis mechanisms with schematic representations.
  • To highlight the application of thiolated polymers in tissue engineering.

Main Methods:

  • Thiolation of natural polymers (collagen, gelatin) using Traut's reagent.
  • Thiolation of carboxylic acid-containing polymers (hyaluronic acid, heparin) via EDC/NHS coupling with cysteamine or L-cysteine.
  • Hydrogel fabrication through disulfide bond formation, thiol-ene, or Michael-type addition reactions.
  • Characterization using Ellman's assay to determine the degree of thiolation.

Main Results:

  • Thiolated polymers exhibit biocompatibility and cellular mimicking properties in vitro.
  • Developed hydrogel scaffolds effectively support proliferation and differentiation of various cell types.
  • Thiolated nanomaterials show potential as crosslinking agents for 3D tissue-engineered hydrogels.

Conclusions:

  • Thiolated polymers, including protein-based, carbohydrate-based, and synthetic types, offer versatile platforms for hydrogel development.
  • The reviewed methods enable the creation of advanced hydrogel scaffolds with promising applications in regenerative medicine.
  • Future research directions include exploring novel thiolated nanomaterials for enhanced tissue engineering.