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Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
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Extracellular chloride is required for efficient platelet aggregation.

Kirk A Taylor1, Darren G S Wilson1, Matthew T Harper2

  • 1a Department of Biomedical and Forensic Sciences , Anglia Ruskin University , Cambridge , UK.

Platelets
|July 21, 2017
PubMed
Summary
This summary is machine-generated.

Extracellular chloride influences platelet activation by affecting aggregation rates and secondary signaling. Anion channels play a role in efficient platelet responses, particularly alpha-granule release.

Keywords:
ADPaggregationchlorideion channels plateletsthrombin

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Area of Science:

  • Biochemistry
  • Hematology
  • Physiology

Background:

  • Anion channels are crucial for various cellular functions, including ATP release and volume regulation.
  • Platelets express multiple anion channels, but their specific roles in platelet activation remain unclear.
  • Lack of specific pharmacological inhibitors necessitates alternative methods to study anion channel function in platelets.

Purpose of the Study:

  • To investigate the functional role of extracellular chloride, and by extension anion channels, in platelet activation.
  • To elucidate the impact of chloride concentration on platelet aggregation dynamics and secondary signaling.
  • To determine the effect of chloride on platelet granule secretion.

Main Methods:

  • Platelet activation was assessed using aggregometry and flow cytometry.
  • Extracellular chloride concentration was manipulated by substitution.
  • Secondary signaling pathways were investigated using aspirin, apyrase, and P2Y12 blockade.

Main Results:

  • Reduced maximum aggregation response to thrombin or collagen-related peptide in the absence of chloride.
  • Significantly decreased aggregation rate dependent on extracellular chloride concentration.
  • Biphasic aggregation in chloride-free conditions indicated impaired secondary signaling.
  • Chloride-dependent alpha-granule release, but not dense granule release, was observed.

Conclusions:

  • Extracellular chloride, and thus anion channels, are important for efficient platelet activation.
  • Anion channels likely enhance platelet secondary signaling pathways.
  • Chloride influences both the rate and extent of platelet aggregation and granule release.