Using Au@nano-C60 nanocomposite as an enhanced sensing platform in modeling a TNT aptasensor

Based on the unique characteristics of the combination of fullerene and gold nanoparticles, we successfully designed a new and facile nanocomposite (Au@nano-C60) to fabricate an aptasensor for the ultra-sensitive and selective detection of TNT. The gold nanoparticles decorated fullerene onto a glassy carbon electrode was prepared using an electrochemical method by the in situ generation of Au nanoparticles onto the surface of the glassy carbon electrode modified with activated fullerene. Successively, the NH₂-Apt as a receptor molecule of 2,4,6-Trinitrotoluen was covalently attached onto the modified electrode surface with the resultant nanocomposite. With the addition of the target onto the aptasensor surface and the formation of target/Apt complex, a linear response was obtained from 0.50 fM to 5 μM as well as a limit of detection down to 0.17 fM. The proposed aptasensor shows a wider linear response range and lower limit of detection for the specific detection of 2,4,6-Trinitrotoluen. This newly developed strategy will pave the way to partly meet the requirements in the field of homeland security and public safety.