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Fixation and Sectioning

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Related Experiment Video

Updated: Feb 26, 2026

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY

Published on: June 30, 2016

Advances and perspectives in tissue clearing using CLARITY.

Kristian H Reveles Jensen¹, Rune W Berg¹

¹University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark.

Journal of Chemical Neuroanatomy

|July 22, 2017

Summary

Tissue clearing methods like CLARITY enable 3D imaging of large biological samples. Recent advances simplify and improve this technique, aiding researchers in selecting optimal protocols for future studies.

Keywords:

1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (PubChem CID: 15908)2,2′-Thiodiethanol (PubChem CID: 5447)Acrylamide (PubChem CID: 6579)Biocytin Brain CLARITY D-Sorbitol (PubChem CID: 5780)Diatrizoic acid (PubChem CID: 2140)Gylcerol (PubChem CID: 753)Histology Immunohistochemistry Iohexol (PubChem CID: 3730)Iomeprol-d3 (PubChem CID: 46781978)N,N,N’,N’-Tetrakis(2-hydroxypropyl)-ethylenediamine (amino alcohol, PubChem CID: 7615)Refractive index Tissue clearing Tracing Virus α-Thioglycerol (PubChem CID: 7291)

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Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO

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Last Updated: Feb 26, 2026

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY

Published on: June 30, 2016

Imaging and Quantification of Intact Neuronal Dendrites via CLARITY Tissue Clearing

Imaging and Quantification of Intact Neuronal Dendrites via CLARITY Tissue Clearing

Published on: April 20, 2021

Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO

Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO

Published on: July 7, 2014

Area of Science:

Neuroscience
Biotechnology
Microscopy

Background:

CLARITY enables deep tissue imaging and 3D reconstruction.
Initial CLARITY protocols were time-consuming and expensive.
Lipid removal via electrophoresis was a key step for transparency.

Purpose of the Study:

Review advances in tissue clearing techniques.
Compare current applications of CLARITY and its variants.
Highlight areas for future methodological improvements.

Main Methods:

Literature review of tissue clearing advancements.
Comparative analysis of different CLARITY protocols.
Discussion of passive clearing (PACT) and improved staining methods.

Main Results:

Several improvements and simplifications have emerged since the initial CLARITY method.
Passive clearing and enhanced staining techniques offer alternatives.
Current applications vary, necessitating careful protocol selection.

Conclusions:

Tissue clearing technology has evolved significantly.
Further improvements are needed for broader accessibility and efficiency.
Guidance is provided for selecting appropriate protocols for specific research needs.