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Nano-antenna enhanced two-focus fluorescence correlation spectroscopy.

Lutz Langguth1, Agata Szuba2, Sander A Mann1

  • 1Center for Nanophotonics, AMOLF, Science Park 102, Amsterdam, NL-1098XG, The Netherlands.

Scientific Reports
|July 22, 2017
PubMed
Summary
This summary is machine-generated.

We introduce two-focus fluorescence correlation spectroscopy (2fFCS) using plasmonic nanoantennas for precise diffusion measurements. Nanoslits enhance cross-correlation signals, enabling accurate diffusion constant determination in lipid bilayers.

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Area of Science:

  • Plasmonics
  • Nanophotonics
  • Biophysics

Background:

  • Fluorescence correlation spectroscopy (FCS) is a powerful technique for studying molecular dynamics.
  • Accurate calibration of diffusion constants in FCS typically requires precise knowledge of the observation volume.
  • Plasmonic nanoantennas offer unique optical properties for enhancing spectroscopic techniques.

Purpose of the Study:

  • To develop and validate two-focus fluorescence correlation spectroscopy (2fFCS) enhanced by plasmonic nanoantennas.
  • To investigate nanoslit geometries as suitable nanoantennas for 2fFCS.
  • To demonstrate the application of this technique for measuring lipid diffusion in supported lipid bilayers.

Main Methods:

  • Electromagnetic modeling of plasmonic nanoantenna geometries (nanorods and nanoslits).
  • Theoretical analysis of two-focus fluorescence correlation spectroscopy (2fFCS) principles.
  • Experimental proof-of-principle using a periodic array of nanoslits and lipid diffusion measurements.

Main Results:

  • Plasmonic nanorods were found unsuitable for 2fFCS due to high background signals.
  • Nanoslit geometries demonstrated potential for distinct cross-correlation signals in orthogonally polarized detection channels.
  • Periodic arrays of nanoslits showed enhanced cross-correlation amplitude.
  • Lipid diffusion in a supported lipid bilayer was successfully measured using the developed 2fFCS technique.

Conclusions:

  • Plasmonic nanoantenna-enhanced 2fFCS, particularly with nanoslits, offers an intrinsically calibrated method for determining diffusion constants.
  • The proposed technique provides a robust approach for studying molecular dynamics in complex biological systems.
  • This method advances the capabilities of fluorescence correlation spectroscopy for nanoscale investigations.