Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Plasma membrane proton ATPase from human kidney.

A L Sallman, H J Lubansky, Z Talor

    European Journal of Biochemistry
    |June 16, 1986
    PubMed
    Summary

    Human kidney plasma membranes contain a proton ATPase (H+-ATPase) involved in urinary acidification. This enzyme

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Efficacy of chewed vs. crushed lanthanum on phosphorus binding in healthy volunteers.

    Clinical nephrology·2010
    Same author

    Oral rapamycin attenuates atherosclerosis without affecting the arterial responsiveness of resistance vessels in apolipoprotein E-deficient mice.

    Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2009
    Same author

    The case: 69 year old man with sand in the urine. N-acetylsulfadiazine crystals.

    Kidney international·2007
    Same author

    Cinacalcet HCI (Sensipar/Mimpara) is an effective chronic therapy for hemodialysis patients with secondary hyperparathyroidism.

    Clinical nephrology·2007
    Same author

    Acid-base disturbance.

    Respiratory care·2001
    Same author

    SFKs, Ras, and the classic MAPK pathway couple muscarinic receptor activation to increased Na-HCO(3) cotransport activity in renal epithelial cells.

    American journal of physiology. Renal physiology·2001

    Area of Science:

    • Nephrology
    • Cell Biology
    • Biochemistry

    Background:

    • Urinary acidification is crucial for maintaining acid-base balance.
    • Proton pumps, specifically proton ATPase (H+-ATPase), are hypothesized to mediate this process.

    Purpose of the Study:

    • To isolate and characterize the H+-ATPase in human kidney plasma membranes.
    • To investigate the role of this enzyme in urinary acidification.

    Main Methods:

    • Isolation of plasma membrane fractions from human kidney cortex and medulla.
    • Assay of ATPase activity and acridine orange fluorescence quenching to detect acidification.
    • Inhibition studies using specific ATPase inhibitors and ionophores.

    Main Results:

    • A plasma membrane fraction enriched in H+-ATPase activity was isolated from human kidney cortex and medulla.
    • This H+-ATPase activity was sensitive to specific inhibitors (DCCD, NEM, vanadate) and utilized ATP.
    • The enzyme was shown to be electrogenic and dependent on chloride, confirming its role in acidification.

    Conclusions:

    • Human kidney cortex and medulla plasma membranes contain a functional H+-ATPase.
    • This H+-ATPase is likely responsible for distal urinary acidification.
    • The findings provide molecular evidence for the mechanism of renal acid excretion.

    Related Experiment Videos