Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and Characterization of CD44-Targeted X-Aptamers with Enhanced Binding Affinity for Cancer Therapeutics.

Bioengineering (Basel, Switzerland)·2025
Same author

Selection and Characterization of Vimentin-Binding Aptamer Motifs for Ovarian Cancer.

Molecules (Basel, Switzerland)·2021
Same author

Conversion of RNA Aptamer into Modified DNA Aptamers Provides for Prolonged Stability and Enhanced Antitumor Activity.

Journal of the American Chemical Society·2021
Same author

Improving vascular maturation using noncoding RNAs increases antitumor effect of chemotherapy.

JCI insight·2021
Same author

Heightened consciousness and curriculum in a time of crisis.

Prospects·2021
Same author

Environmental salinity influences the branchial expression of TCR pathway related genes based on transcriptome of a catadromous fish.

Comparative biochemistry and physiology. Part D, Genomics & proteomics·2021

Related Experiment Video

Updated: Feb 26, 2026

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

25.5K

X-Aptamer Selection and Validation.

Ganesh L Lokesh1,2, Hongyu Wang1,2, Curtis H Lam3

  • 1Institute of Molecular Medicine for the Prevention of Human Diseases, The University of Texas Health Science Center, 1825 Pressler Street, Houston, TX, 77030, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 22, 2017
PubMed
Summary

X-Aptamers offer superior molecular targeting with unlimited functional groups compared to traditional aptamers. This discussion focuses on the streamlined X-Aptamer selection process for enhanced biomarker discovery and diagnostics.

Keywords:
AptamerAptamer nanoparticle conjugationAptamer siRNA chimerasBead-based selectionBiotin labelingChemical cross-linkingDrug aptamer conjugationDye labelingMolecular targetingProteomicsSplit-pool synthesisX-Aptamer

More Related Videos

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
12:23

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

2.1K
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.7K

Related Experiment Videos

Last Updated: Feb 26, 2026

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

25.5K
In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
12:23

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

2.1K
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.7K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Chemical Biology

Background:

  • Aptamers and analogs like X-Aptamers are crucial for molecular targeting, biomarker discovery, and disease diagnosis.
  • Current aptamer selection (SELEX) is time-consuming and yields limited functional groups.
  • X-Aptamers present a significant advancement with an unlimited number of functional groups.

Purpose of the Study:

  • To discuss the X-Aptamer selection process.
  • To highlight X-Aptamers as superior targeting agents compared to traditional aptamers.
  • To emphasize the advantages of X-Aptamers in molecular pathway targeting and diagnostics.

Main Methods:

  • Discussion of the X-Aptamer selection methodology.
  • Comparison of X-Aptamer selection with traditional SELEX.
  • Focus on the process enabling unlimited functional groups.

Main Results:

  • X-Aptamer selection offers a more efficient and versatile approach.
  • Unlimited functional groups enhance targeting capabilities.
  • The process facilitates advanced applications in diagnostics and therapeutics.

Conclusions:

  • X-Aptamers are superior targeting agents due to their unlimited functional groups.
  • The X-Aptamer selection process is more advantageous than traditional SELEX.
  • This technology holds significant promise for molecular targeting, biomarker discovery, and disease diagnosis.