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Published on: February 1, 2019
Preparation and Optimization of Lipid-Like Nanoparticles for mRNA Delivery
1Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, The Ohio State University, 496 W. 12th Ave., Columbus, OH, 43210, USA.
Abstract:
Lipid-like nanoparticles (LLNs) have shown great promise for nucleic acid delivery. Recently, we have developed N 1,N 3,N 5-tris(2-aminoethyl)benzene-1,3,5-tricarboxamide (TT) derived lipid-like compounds, formulated them into TT LLNs for mRNA delivery, and applied an orthogonal array design to facilitate formulation optimization. This chapter focuses on the following contents relevant to lipid-like nanoparticles: formulation method, particle characterization, orthogonal array design, and in vitro assays.
