Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical Application of Eyelid Composite Tissue Flap in the Repair of Full-thickness Eyelid Margin Defects.

Plastic and reconstructive surgery. Global open·2026
Same author

[Petroleum ether fraction of <i>Myrica nana</i> roots relieves spasmodic abdominal pain in mice by inhibiting intestinal smooth muscle spasm].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University·2026
Same author

Risk-managed safety screening via stability-constrained reachability tubes for vehicle handling.

Scientific reports·2026
Same author

Vehicle-Conditional Split-Conformal Calibration for Risk-Budgeted Sub-Second Proxy-Triggered Vehicle Instability Warnings from Past-Only Sensor Slices.

Sensors (Basel, Switzerland)·2026
Same author

A Dynamic Neck Mass in an Infant: Expanding the Differential.

Pediatrics in review·2026
Same author

Hepatic SEC16B regulates lipid homeostasis by coordinating VLDL secretion and lipid droplet expansion.

The Journal of clinical investigation·2026

Related Experiment Video

Updated: Feb 26, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

448

Polycationic Probe-Guided Nanopore Single-Molecule Counter for Selective miRNA Detection.

Kai Tian1,2, Ruicheng Shi1,2, Amy Gu1,2

  • 1Department of Bioengineering, University of Missouri, 134 Research Park, Columbia, MO, 65211, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 22, 2017
PubMed
Summary

This study introduces a novel peptide-PNA probe method for highly accurate microRNA (miRNA) detection using nanopore sensors. The technique effectively filters out interfering nucleic acids, enabling sensitive and specific miRNA biomarker discovery.

Keywords:
BiosensorCancerDiagnosticsHIV-1 TAT peptideNanoporeNucleic acidsPNAProbeSingle moleculemiRNA (miRNA)

More Related Videos

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

34.0K
Author Spotlight: Advancements in DNA Nanosensors &#8211; Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.6K

Related Experiment Videos

Last Updated: Feb 26, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

448
Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

34.0K
Author Spotlight: Advancements in DNA Nanosensors &#8211; Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.6K

Area of Science:

  • Biomarkers and Diagnostics
  • Nanotechnology in Medicine
  • Molecular Biology

Background:

  • MicroRNAs (miRNAs) are crucial noncoding RNAs with potential as disease biomarkers.
  • Nanopore single-molecule sensing offers a promising noninvasive method for miRNA detection.
  • Nontarget nucleic acids in biofluid samples create "contaminative" signals, hindering accurate miRNA detection.

Purpose of the Study:

  • To develop a novel method for specific and sensitive detection of target microRNAs (miRNAs) using nanopore technology.
  • To overcome the challenge of signal interference from nontarget nucleic acids in biofluid samples.
  • To enable accurate miRNA detection for diagnostics and prognosis applications.

Main Methods:

  • Utilized a designed polycationic peptide-PNA probe to specifically guide target miRNA towards the nanopore.
  • Developed a mechanism to reject nontarget nucleic acids lacking the probe, preventing signal contamination.
  • Employed PNA's specificity for capturing miRNAs to discriminate even single-nucleotide differences.

Main Results:

  • Achieved specific guidance of target miRNAs to the nanopore using the peptide-PNA probe.
  • Successfully rejected nontarget nucleic acids, significantly reducing signal interference.
  • Demonstrated the ability to detect target miRNAs at low concentrations with high accuracy.
  • Showcased discrimination of miRNAs with single-nucleotide variations.

Conclusions:

  • The developed peptide-PNA probe method significantly enhances the accuracy and sensitivity of nanopore-based miRNA detection.
  • This approach effectively addresses the challenge of nontarget nucleic acid interference in biofluid samples.
  • The protocol provides a detailed procedure for future miRNA detection in clinical diagnostics and prognosis.