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Updated: Feb 26, 2026
An Efficient Protocol to Assess ERK Activity Modulation in Early Zebrafish Noonan Syndrome Models via Live FRET Microscopy and Immunofluorescence
Published on: May 2, 2025
Single-Cell Imaging of ERK Signaling Using Fluorescent Biosensors
Michael Pargett1, Taryn E Gillies1, Carolyn K Teragawa1
1Department of Molecular and Cellular Biology, University of California, Davis, 243 Briggs Hall, One Shields Avenue, Davis, CA, 95616, USA.
Abstract:
Single-cell analysis of the mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase (ERK) provides a means to perform highly detailed kinetic studies, assess heterogeneity between cells, and distinguish the subcellular localization of ERK activity. We describe here the methods needed to perform such measurements in a cell type of the investigator's choosing. We discuss the selection of appropriate reporters and provide detailed methods for stably introducing reporters, collecting live-cell data, and automatically extracting quantitative information from individual cells.
