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Topoisomerase I deregulation in laryngeal squamous cell carcinomas based on tissue microarray analysis
Theodoros A Papadas1, Evangelos Tsiambas, Nicholas S Mastronikolis
1Department of Otorhinolaryngology, Head and Neck Surgery, Medical School, University of Patras; Patras, Greece.
Summary
Topoisomerase I (Topo I) is overexpressed in 64% of laryngeal squamous cell carcinomas (LSCC), correlating with tumor differentiation. This finding suggests Topo I as a potential therapeutic target in LSCC patients.
Area of Science:
- Molecular Biology
- Oncology
- Genetics
Background:
- Topoisomerases are crucial enzymes regulating DNA topology during essential cellular processes.
- Aberrant Topoisomerase I (Topo I) expression is implicated in various solid malignancies.
- Topo I inhibitors are established chemotherapeutic agents, highlighting its role in cancer treatment.
Purpose of the Study:
- To investigate the expression patterns of Topoisomerase I (Topo I) protein in laryngeal squamous cell carcinomas (LSCC).
- To determine the correlation between Topo I expression and clinicopathological features of LSCC, particularly tumor differentiation.
Main Methods:
- Utilized tissue microarray (TMA) technology for analyzing 50 primary LSCC samples.
- Employed immunohistochemistry with anti-Topo I antibody to assess protein expression levels.
- Implemented digital image analysis for objective quantification of Topo I staining in tumor cell nuclei.
Main Results:
- Topo I protein overexpression was detected in 64% (32/50) of LSCC cases.
- Low Topo I expression was observed in 36% (18/50) of the samples.
- A significant association was found between Topo I expression levels and the grade of tumor differentiation (p=0.021).
Conclusions:
- Topo I overexpression is a frequent event in a significant proportion of LSCC.
- The level of Topo I expression is linked to the differentiation status of laryngeal tumors.
- Further research into the mechanisms of Topo I deregulation is warranted to guide targeted therapy selection in LSCC.
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