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Related Experiment Video

Updated: Feb 26, 2026

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
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miR-181d regulates human dendritic cell maturation through NF-κB pathway.

Xian Wei Su1, Gang Lu1, Chi Kwan Leung2

  • 1Division of Neurosurgery, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, China.

Cell Proliferation
|July 22, 2017
PubMed
Summary
This summary is machine-generated.

MicroRNA-181d promotes dendritic cell maturation by activating the NF-κB pathway. This involves targeting CYLD, enhancing immune responses and T-cell proliferation.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • MicroRNAs (miRNAs) are key post-transcriptional gene regulators in cellular processes.
  • Dendritic cells (DCs) play crucial roles in immunity and development.
  • Understanding miRNA functions in DC maturation is vital for immune regulation.

Purpose of the Study:

  • To investigate the function of miR-181d in dendritic cell (DC) maturation.
  • To elucidate the underlying molecular mechanisms of miR-181d in DCs.
  • To explore the impact of miR-181d on immune cell interactions.

Main Methods:

  • miRNA screening in human DCs using quantitative real-time PCR (qRT-PCR).
  • Analysis of DC maturation markers (CD80, CD83) post-miRNA mimic transfection.
  • Investigation of signaling pathways (NF-κB) using pharmacological inhibitors and Western blot.
  • Luciferase assay and mixed lymphocyte reaction (MLR) to identify targets and assess T-cell responses.

Main Results:

  • Overexpression of miR-181d promotes DC maturation and up-regulates CD80 and CD83.
  • miR-181d activates the NF-κB pathway, increasing IL-12 and TNF-α expression.
  • miR-181d targets cylindromatosis (CYLD), a negative regulator of NF-κB.
  • miR-181d-transfected DCs enhance T-cell proliferation in vitro.

Conclusions:

  • miR-181d is essential for DC maturation.
  • miR-181d activates the NF-κB pathway by targeting CYLD.
  • This mechanism highlights miR-181d's role in modulating immune responses via DCs.