Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetic Screens02:46

Genetic Screens

5.8K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
5.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synaptic activity controls local exposure of an 'eat-me' signal via ANO3-ITPR1 signaling.

bioRxiv : the preprint server for biology·2026
Same author

Knowledge, Attitudes and Practices Related to Antimicrobial Use and Resistance Among Livestock Sector Stakeholders in Seven Former Soviet Countries: A Multi-Country Regional Analysis.

Antibiotics (Basel, Switzerland)·2026
Same author

Investigating the analytical robustness of the social and behavioural sciences.

Nature·2026
Same author

Enhanced Tumor-to-Background Contrast with [<sup>52</sup>Mn]Mn-BPPA-Bevacizumab VEGF-Targeted Immuno-PET in Cervical Cancer.

Pharmaceuticals (Basel, Switzerland)·2026
Same author

In vivo Proximity & Spatial Proteomics with CRISPR Screening Identify STXBP1 as a Protective Modifier of α-synuclein Toxicity in Dopamine Neurons.

bioRxiv : the preprint server for biology·2026
Same author

Evaluating the Impact of Prophylactic Administration of Ivermectin on the Fecal Microbiome of Healthy C57BL/6J Mice (Mus musculus).

Journal of the American Association for Laboratory Animal Science : JAALAS·2026

Related Experiment Video

Updated: Feb 26, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

23.4K

CRISPulator: a discrete simulation tool for pooled genetic screens.

Tamas Nagy1, Martin Kampmann2,3

  • 1Graduate program in Bioinformatics, University of California, San Francisco, CA, 94158, USA.

BMC Bioinformatics
|July 23, 2017
PubMed
Summary

CRISPulator, a new tool, simulates CRISPR genetic screens to optimize experimental design. This computational approach saves time and resources by predicting the impact of various parameters on pooled screen results.

Keywords:
CRISPRCRISPRiFunctional genomicsGenome-wide screensMonte CarloSimulation

More Related Videos

Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays
11:05

Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays

Published on: January 7, 2019

10.0K
Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens
09:33

Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens

Published on: August 25, 2023

1.7K

Related Experiment Videos

Last Updated: Feb 26, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

23.4K
Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays
11:05

Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays

Published on: January 7, 2019

10.0K
Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens
09:33

Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens

Published on: August 25, 2023

1.7K

Area of Science:

  • CRISPR gene editing
  • Genomic screening
  • Computational biology

Background:

  • CRISPR technology enables pooled genetic screens in biomedical research.
  • Screen design parameters significantly impact pooled screen quality, cost, and scalability.
  • Experimental testing of numerous parameters is often prohibitive due to cost and effort.

Purpose of the Study:

  • To develop a computational tool for optimizing pooled CRISPR genetic screen design.
  • To enable researchers to explore a wide range of experimental parameters in silico.
  • To inform experimental strategies and improve the robustness of screen results.

Main Methods:

  • Development of CRISPulator, a Monte Carlo simulation tool.
  • Supports simulation of CRISPR interference (CRISPRi) and CRISPR nuclease (CRISPRn) screens.
  • Accommodates screens based on cell growth/survival and fluorescence-activated cell sorting phenotypes.

Main Results:

  • CRISPulator simulates the impact of screen parameters on result robustness.
  • Provides users with intuition and insights for experimental strategy development.
  • Facilitates in silico exploration of a large parameter space for pooled screens.

Conclusions:

  • CRISPulator streamlines pooled genetic screen design by reducing experimental trial and error.
  • Enables cost-effective optimization of screen parameters.
  • Derives non-obvious rules for optimal pooled CRISPR screen design.