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Updated: Feb 26, 2026
Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications
Published on: April 13, 2022
Surface behavior and bulk properties of aqueous chitosan and type-B gelatin solutions for effective emulsion
Jagadish Chandra Roy1, Fabien Salaün2, Stéphane Giraud2
1University Lille Nord de France, F-5900 Lille, France; ENSAIT, GMTEX, F-59100, Roubaix, France; Department of Applied Science and Technology, Politecnico di Torino, Italy; College of Textile and Clothing Engineering, Soochow University, Suzhou, Jiangsu, 215123, China.
Abstract:
The behavior of aqueous chitosan (CH), type-B gelatin (GB) and CH-GB coacervate was studied on oil-in-water emulsion formulation at various pH and concentration ratio. The coacervate was formed by phase separation at ratios CH:GB, 1:10 to 1:1 with total biopolymer concentrations of 0.55%-1.0% (w/v) at pH 4.0-5.5. Soluble complexes were formed below pH 5.0 and coacervate formation was confirmed at pH 5.0 and above by zeta potential and UV-spectroscopy measurements. The coacervate formation was found maximum at the CH-GB ratios of 1:10 and 1:5 at pH 5.5. Formulated emulsions (>10μm droplets) using 1% (w/v) chitosan and GB were found stable (+52.5mv and creaming index 86%) and unstable respectively. Emulsion stabilized by mixed CH:GB 1:5 (3%w/v) had no creaming effect. The instability was attributed to the lower surface activity (K=5.0Lg-1) of pure GB compared to CH (K=14.3Lg-1). The formulation and methods can successfully tune the stability of the emulsions.
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