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Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format
Published on: September 17, 2016
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Transformation of Schizosaccharomyces pombe in a 96-Well Format
Assen Roguev1, Jiewei Xu2, Nevan J Krogan2
1Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California 94518 roguev@gmail.com.
Cold Spring Harbor Protocols
|July 23, 2017
Abstract:
This protocol describes chemical transformation of Schizosaccharomyces pombe with linear DNA in a 96-well format. This procedure has been successfully used for large-scale strain construction in fission yeast.
