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Related Experiment Video

Updated: Feb 26, 2026

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells MSCs
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Steroid-Mediated Decrease in Blood Mesenchymal Stem Cells in Liver Transplant could Impact Long-Term Recovery.

Nykia D Walker1,2, Yasmine Mourad2, Katherine Liu3

  • 1Department of Medicine, Division of Hematology/Oncology, Rutgers New Jersey Medical School, Newark, NJ, 07103, USA.

Stem Cell Reviews and Reports
|July 23, 2017
PubMed
Summary
This summary is machine-generated.

Glucocorticoids (GC) significantly decrease mesenchymal stem cells (MSCs) in liver transplant recipients. This impacts MSC function and recovery, suggesting a need to re-evaluate GC use and explore cell therapy alternatives.

Keywords:
ChemokineGlucocorticoidInflammationLiverMesenchymal stem cellsRegenerative medicineStem cellSteroidTransplant

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Area of Science:

  • Immunology
  • Regenerative Medicine
  • Transplantation Science

Background:

  • Orthotopic liver transplant (OLT) is standard for end-stage liver disease, often requiring immunosuppression with glucocorticoids (GC).
  • Mesenchymal stem cells (MSCs) possess regenerative and immune-modulatory properties, crucial for post-transplant recovery.
  • The impact of GC on endogenous MSCs in OLT recipients is not fully understood.

Purpose of the Study:

  • To investigate the effects of GC on circulating MSCs in OLT recipients.
  • To elucidate the in vitro mechanisms by which GC affect MSC migration and function.
  • To assess the clinical implications of GC-induced MSC alterations in OLT.

Main Methods:

  • Phenotypic analysis of blood samples from OLT recipients at various post-transplant time points.
  • In vitro transwell migration assays using MSCs and GC-treated liver tissue.
  • Assessment of MSCs' CXCL12/receptor axis expression and cytoskeletal dynamics.

Main Results:

  • GC administration led to a significant decrease in circulating MSCs in OLT recipients.
  • MSCs showed impaired migration towards GC-exposed liver tissue and reduced response to CXCL12 in vitro.
  • GC-treated MSCs exhibited cytoskeletal retraction and decreased motility, linked to the CXCL12/receptor axis.
  • MSC levels recovered post-transplant, but migratory function remained suboptimal.

Conclusions:

  • GC use in OLT is associated with a reduction in functional MSCs, potentially impairing regenerative processes.
  • The observed MSC dysfunction is partly due to alterations in the CXCL12/receptor pathway.
  • Findings suggest a need to re-evaluate GC regimens in OLT and consider adjuvant cell therapies to mitigate negative effects.