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Updated: Feb 26, 2026

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

Fast multiplex analysis of antibodies in complex sample matrix using the microfluidic Evalution™ platform.

Karen Leirs¹, Pelin Leblebici¹, Jeroen Lammertyn¹

¹KU Leuven, BIOSYST-MeBioS, Willem de Croylaan 42, 3001, Heverlee, Belgium.

Analytica Chimica Acta

|July 24, 2017

Summary

The Evalution™ microfluidic platform enables rapid, high-throughput optimization of biosensor assays. It efficiently evaluates antibody performance for influenza A nucleoprotein detection in various conditions and sample types.

Keywords:

Barcoded microparticles Bioassay development Influenza nucleoprotein Microfluidic evalution platform Multiplex analysis

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Last Updated: Feb 26, 2026

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Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Published on: October 10, 2013

Area of Science:

Biomedical Engineering
Analytical Chemistry
Immunotechnology

Background:

Biosensor development demands optimization of bioassay conditions for kinetics, sensitivity, and specificity.
Existing methods for studying bioreceptor-target interactions often suffer from long hands-on times, low throughput, high sample consumption, and elevated costs.

Purpose of the Study:

To evaluate the novel microfluidic Evalution™ platform for developing fast, high-throughput sandwich-based assays.
To optimize bioassay parameters and assess antibody performance for influenza A nucleoprotein detection.

Main Methods:

Utilized the Evalution™ platform to test various bioassay parameters, including non-specific binding, reagent concentrations, and incubation times, for three capture antibodies.
Performed optimization runs in 80-minute intervals.
Applied optimized conditions for detecting influenza A nucleoprotein in buffer and nasopharyngeal swabs.

Main Results:

The Evalution™ platform facilitated rapid assessment of antibody performance.
Antibody Ab66 demonstrated the highest sensitivity (LOD of 0.04 ng mL⁻¹) with a 30-minute incubation, while Ab11 and Ab12 required 60 minutes for sensitive detection.
Antibody Ab12 exhibited limited capture capacity in complex sample matrices.

Conclusions:

The Evalution™ platform is a reliable tool for rapid, multiplexed analysis of antibody performance in both buffer and complex biological samples.
The platform's efficiency supports faster biosensor development and optimization.