Related Experiment Video

Updated: Feb 26, 2026

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

7.7K

MiR-143 regulates the proliferation and migration of osteosarcoma cells through targeting MAPK7

Xiancheng Dong1, Bin Lv2, Yusong Li3

  • 1Nanjing Medical University, Nanjing, PR China; Department of Orthopedics, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, PR China.

Archives of Biochemistry and Biophysics
|July 24, 2017
PubMed

Insights

MicroRNA-143 (miR-143) acts as a tumor suppressor in Osteosarcoma (OS). Lower miR-143 levels correlate with increased OS cell proliferation and invasion, suggesting miR-143 as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA-143 (miR-143) is implicated as a tumor suppressor in various cancers.
  • The specific role and mechanism of miR-143 in Osteosarcoma (OS) pathogenesis require further elucidation.
  • Current Osteosarcoma prognosis remains suboptimal, necessitating novel therapeutic strategies.

Purpose of the Study:

  • To investigate the expression levels of miR-143 in Osteosarcoma tissues and cell lines.
  • To explore the functional role of miR-143 in Osteosarcoma initiation and progression.
  • To identify the molecular targets and mechanisms underlying miR-143's function in Osteosarcoma.

Main Methods:

  • Quantitative analysis of miR-143 expression in clinical Osteosarcoma samples and cell lines.
  • Gain-of-function experiments to assess the impact of miR-143 overexpression on OS cell behavior.
  • Bioinformatics analysis and luciferase reporter assays to validate target genes.

Main Results:

  • miR-143 expression was significantly downregulated in Osteosarcoma tissues and cells compared to normal controls.
  • Forced expression of miR-143 suppressed Osteosarcoma cell proliferation, migration, and invasion.
  • Mitogen-activated protein kinase 7 (MAPK7) was identified as a direct target gene of miR-143.

Conclusions:

  • miR-143 functions as a tumor suppressor in Osteosarcoma.
  • Downregulation of miR-143 contributes to Osteosarcoma tumorigenesis.
  • miR-143, potentially through targeting MAPK7, represents a promising therapeutic target for Osteosarcoma treatment.
Keywords:
MAPK7MigrationOsteosarcomaProliferationmiR-143

More Related Videos

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

11.6K
A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells11:15

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Published on: May 6, 2018

10.7K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.9K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.8K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
8.8K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.3K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.4K