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Updated: Feb 26, 2026

Assaying for Inorganic Polyphosphate in Bacteria
Published on: January 21, 2019
Study of Acid Phosphatase in Solubilization of Inorganic Phosphates by Piriformospora indica
Swetha Seshagiri1, Padmavathi Tallapragada2
1Department of Microbiology, Centre for PG Studies, Jain University, Bangalore, India </br> Centre for Emerging Technologies TM, Jain University, Ramanagara, Bangalore, India.
Abstract:
Phosphorus is an essential plant macronutrient present in the soil. Only a small portion of phosphorus in soil is taken up by plants and the rest of it becomes unavailable to plants as it is immobilized. Phosphate solubilizing microorganisms play a vital role in converting the insoluble form of phosphates to the soluble form. The present paper reports the solubilization of tricalcium phosphate, rock phosphate, single super phosphate, zinc phosphate and aluminum phosphate by Piriformospora indica with the production of organic acids as well as acid phosphatase. The amount of phosphate released (4.73 mg ml-1) and titratable acidity (0.12%) was found to be the highest in the case of single super phosphate as compared to other phosphate sources. High performance liquid chromatography (HPLC) revealed the presence of oxalic acid, lactic acid, citric acid and succinic acid in the media. Highest phosphatase activity was observed with the cell membrane extract of the organism in the presence of zinc phosphate.
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