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Updated: Aug 11, 2026

Particle Agglutination Method for Poliovirus Identification
Published on: April 20, 2011
Detection of Polioviruses in Sewage Using Cell Culture and Molecular Methods
Agnieszka Figas1, Magdalena Wieczorek1, Bogumiła Litwińska1
1National Institute of Public Health - National Institute of Hygiene, Department of Virology, Warsaw, Poland.
Abstract:
The work presented here demonstrates the utility of a two-step algorithm for environmental poliovirus surveillance based on: preselection of sewage samples tested for the presence of enteroviral genetic material-RT-PCR assay and detection of infectious viruses by cell culture technique (L20B for polioviruses and RD for polio and other non-polio enteroviruses). RD and L20B cell lines were tested to determine their sensitivity for isolation of viruses from environmental samples (sewage). Finally, we wanted to determine if sewage concentration affects the results obtained for RT-PCR and cell cultures.

