Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanistic Links Between Obesity and Breast Cancer Progression: Cellular Crosstalk, Metabolic Reprogramming and Microenvironmental Drivers.

Cell biochemistry and function·2026
Same author

Protocol: A multi-factorial, multi-centre study, for biomarker identification in healthy controls for comparison to babies with moderate-severe NESHIE.

PloS one·2026
Same author

Olfactory and Cognitive Performance Improvement After Oxygen-Ozone Major Autohemotherapy in Mild Cognitive Impairment: A Retrospective Cohort Study.

Neurology international·2026
Same author

Pelvic floor muscle training versus De Gasquet method in non-elite sports women: a pilot trial.

The Journal of sports medicine and physical fitness·2026
Same author

Treatment effectiveness and preference in patients with schizophrenia switched from oral standard of care atypical antipsychotics to aripiprazole once-monthly: a brief report over a six-month period.

Frontiers in psychiatry·2025
Same author

An epigenetic perspective on neonatal encephalopathy with suspected hypoxic ischaemic encephalopathy.

Clinical epigenetics·2025

Related Experiment Video

Updated: Feb 26, 2026

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants
07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

853

AMLprofiler: A Diagnostic and Prognostic Microarray for Acute Myeloid Leukemia.

Marco Alessandrini1, Sharon S Kappala1, Michael S Pepper2

  • 1Faculty of Health Sciences, Department of Immunology, and Institute for Cellular and Molecular Medicine, SAMRC Extramural Unit for Stem Cell Research and Therapy, University of Pretoria, Room 5-64, Level 5, Pathology Building, 5 Bophelo Road, Gezina, Pretoria, South Africa.

Methods in Molecular Biology (Clifton, N.J.)
|July 24, 2017
PubMed
Summary

The AMLprofiler assay accurately predicts survival in acute myeloid leukemia (AML) patients. This diagnostic tool uses RNA analysis to assess cytogenetics, mutation, and expression for personalized treatment strategies.

Keywords:
AMLprofilerAcute myeloid leukemiaMicroarraySkylineDx

More Related Videos

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia
09:57

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Published on: March 5, 2018

30.8K
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
09:01

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

Published on: March 26, 2018

14.8K

Related Experiment Videos

Last Updated: Feb 26, 2026

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants
07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

853
Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia
09:57

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Published on: March 5, 2018

30.8K
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
09:01

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

Published on: March 26, 2018

14.8K

Area of Science:

  • Hematology
  • Molecular Diagnostics
  • Oncology

Background:

  • Acute myeloid leukemia (AML) is a heterogeneous hematologic malignancy characterized by immature myeloid cell proliferation.
  • Genetic abnormalities and patient-specific factors significantly impact treatment response and survival outcomes in AML.
  • Accurate prognostic tools are crucial for tailoring therapeutic strategies and improving patient management.

Purpose of the Study:

  • To evaluate the AMLprofiler, a novel in vitro diagnostic microarray, for predicting post-therapy survival in acute myeloid leukemia (AML) patients.
  • To assess the feasibility and consistency of the AMLprofiler assay in a clinical laboratory setting.

Main Methods:

  • The AMLprofiler assay utilizes RNA chemistry with seven assays across cytogenetics, mutation, and expression analysis.
  • Bone marrow samples are processed within 48 hours of collection, using Affymetrix GeneChip technology.
  • Data analysis is performed on the Affymetrix GeneChip 3000Dx v2 system, with results generated within 15 minutes.

Main Results:

  • The AMLprofiler assay demonstrated consistent data generation compared to standard diagnostic modalities.
  • The assay integrates multiple technologies for a comprehensive molecular profile of AML.
  • Validated for bone marrow samples, ensuring reliable RNA isolation and processing.

Conclusions:

  • The AMLprofiler is a validated qualitative in vitro diagnostic tool for predicting survival in AML patients.
  • The assay's integration of cytogenetic, mutation, and expression analyses offers valuable prognostic information.
  • Consistent performance suggests the AMLprofiler can be reliably implemented in clinical laboratories for improved AML patient care.