Related Experiment Video
Updated: Feb 26, 2026
An Orthotopic Bladder Cancer Model for Gene Delivery Studies
Published on: December 1, 2013
[S100A4, a potential therapeutic target on bladder cancer stem cells]
Chun-Yan Wang1, Qing-Wen Nie, Xuan Zhou
1Department of Neurology, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510315, China.E-mail: 362142518@qq.com.
Objective:
To observe the effect of S100A4 gene silencing mediated by small interfering RNA (siRNA) on the proliferation of bladder cancer stem cells (CSCs) and their capacity of xenograft tumor formation.
Methods:
MB49 bladder cancer stem cells (MCSCs) were isolated and identified. The differentially expressed protein S100A4 was identified in MCSCs using isobaric tags for relative and absolute quantitation technology (iTRAQ). A siRNA targeting S100A4 was constructed and transfected into MCSCs, and its inhibitory effects on S100A4 expression in MCSCs were assessed with Western blotting and qPCR. The effects of siRNA-mediated S100A4 silencing on the proliferation and xenograft tumor formation ability of MCSCs were observed.
Results:
Among the 65 differentially expressed proteins identified by iTRAQ combined with LC/MS/MS, S100A4 protein showed the most distinct differential expression in MCSCs. Transfection of MCSCs with S100A siRNA significantly inhibited the expressions of S100A4 at both mRNA and protein levels, caused obvious suppression of the cell proliferation, and attenuated the xenograft tumor formation ability of the cells in nude mice.
Conclusion:
S100A4 in MCSCs is associated with the recurrence and metastasis of bladder cancer. S100A4 may serve as a potential therapeutic target for eliminating bladder CSCs.
Insights
Small interfering RNA (siRNA) targeting S100A4 gene effectively inhibited bladder cancer stem cell (CSC) proliferation and tumor formation. S100A4 silencing presents a potential therapeutic strategy for bladder cancer treatment.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Context:
- Bladder cancer is a significant health concern, often characterized by recurrence and metastasis.
- Cancer stem cells (CSCs) play a crucial role in tumor initiation, progression, and therapeutic resistance.
- S100A4, a calcium-binding protein, has been implicated in various cancers, but its role in bladder CSCs requires further elucidation.
Purpose:
- To investigate the effect of S100A4 gene silencing using small interfering RNA (siRNA) on the proliferation and xenograft tumor formation of bladder cancer stem cells (CSCs).
Summary:
- S100A4 was identified as a highly differentially expressed protein in MB49 bladder cancer stem cells (MCSCs) using iTRAQ technology.
- Transfection of MCSCs with S100A4-targeting siRNA significantly reduced S100A4 expression at both mRNA and protein levels.
- siRNA-mediated S100A4 silencing suppressed MCSC proliferation and attenuated their ability to form xenograft tumors in vivo.
Impact:
- S100A4 is associated with bladder cancer recurrence and metastasis.
- Targeting S100A4 in bladder CSCs represents a promising therapeutic strategy for eliminating cancer stem cells and potentially preventing disease progression.
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