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Related Concept Videos

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
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High-dynamic-range fluorescence laminar optical tomography (HDR-FLOT).

Qinggong Tang1,2, Yi Liu1,2, Vassiliy Tsytsarev3

  • 1Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742 USA.

Biomedical Optics Express
|July 25, 2017
PubMed
Summary

High-dynamic-range fluorescence laminar optical tomography (HDR-FLOT) enhances imaging depth and concentration range for biological samples. This advancement improves 3D reconstruction accuracy in applications like neuroscience and oncology.

Keywords:
(170.2520) Fluorescence microscopy(170.3010) Image reconstruction techniques(170.3880) Medical and biological imaging(170.6960) Tomography

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Area of Science:

  • Biomedical optics
  • Medical imaging technology

Background:

  • Three-dimensional fluorescence laminar optical tomography (FLOT) offers 100-200 µm resolution and 2-3 mm penetration depth.
  • FLOT is utilized in tissue engineering, neuroscience, and oncology.
  • Current FLOT systems face limitations due to charge-coupled device (CCD) dynamic range, hindering imaging of samples with large concentration differences and limiting penetration depth and quantitative accuracy.

Purpose of the Study:

  • To introduce a high-dynamic-range fluorescence laminar optical tomography (HDR-FLOT) system.
  • To enhance the penetration depth and imaging capabilities for fluorescent samples with significant concentration variations.
  • To improve the quantitative accuracy of 3D reconstruction in FLOT.

Main Methods:

  • Incorporation of the high-dynamic-range (HDR) imaging method into FLOT.
  • Testing the HDR-FLOT system using an agar phantom.
  • In vivo validation of HDR-FLOT for brain imaging in a B6 mouse model.

Main Results:

  • HDR-FLOT demonstrates increased penetration depth compared to conventional FLOT.
  • The system effectively images fluorescent samples with large concentration differences.
  • Improved quantitative accuracy in 3D reconstruction data is achieved.

Conclusions:

  • HDR-FLOT significantly overcomes the dynamic range limitations of traditional FLOT systems.
  • This enhanced technique expands the applicability of FLOT in biological and medical imaging.
  • HDR-FLOT shows promise for advanced 3D in vivo imaging in neuroscience and oncology research.