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Updated: Feb 26, 2026

Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and Transcriptome
Published on: February 24, 2023
Bioinformatics analysis in menopause transition promotes distinct modulation in calvaria and bone marrow osteoblastic
Chenyu Li1, Yanfei Wang1, Hong Luan1
1Department of Nephrology, The Affiliated Hospital of Qingdao University, Qingdao, China.
Abstract:
We read with great interest the recent report by Semeghini et al. (2017), Cell Biol Int, "Menopause transition promotes distinct modulation of mRNAs and miRNAs expression in calvaria and bone marrow osteoblastic cells," which appeared on 24 May 2017 in Cell Biology International. The results of the report are very helpful for us; however, from our perspective, the author's method in bioinformatics analysis is inappropriate: Student's t-test is an inappropriate statistical method for detecting differentially expressed mRNA or miRNA in osteoblastic cells from calvaria of ovariectomized rats compared to control or in osteoblastic cells from bone marrow of ovariectomized rats compared to control.
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