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Circulating leucocytes perpetuate stroke-induced aortic dysfunction.

Shinichi Asano1,2, Grant C O'Connell3, Kent C Lemaster4

  • 1Division of Exercise Physiology, West Virginia University, Morgantown, WV, USA.

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Stroke impacts aortic endothelial function through peripheral circulating leucocytes. Leucocytes stimulated by stroke patient serum impair aortic ring relaxation, highlighting a role for systemic inflammation in vascular dysfunction.

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Area of Science:

  • Cardiovascular Research
  • Immunology
  • Vascular Biology

Background:

  • Post-stroke inflammation is linked to adverse outcomes.
  • Vascular endothelium responds to inflammatory cytokines.
  • Effects of stroke on conduit artery function are not fully understood.

Purpose of the Study:

  • To investigate if stroke influences aortic endothelial function.
  • To determine the role of peripheral circulating leucocytes in stroke-induced aortic vascular reactivity.
  • To test if stroke patient serum triggers leucocyte-dependent aortic endothelial dysfunction.

Main Methods:

  • Isolated total leucocytes from healthy individuals and incubated them in serum from control or stroke patients.
  • Quantified cytokine levels in media using immunoassay.
  • Assessed vascular reactivity of rat aortic rings co-cultured with leucocytes and stimulated with patient or control serum.

Main Results:

  • Aortic endothelium-dependent relaxation was significantly impaired in rings co-cultured with leucocytes and stroke patient serum.
  • No impairment in vascular reactivity was observed in aortic rings without leucocytes.
  • Leucocyte-derived cytokine concentrations increased significantly over time when stimulated with stroke patient serum.

Conclusions:

  • Leucocyte-dependent mechanisms contribute to aortic endothelial dysfunction following stroke.
  • Systemic inflammation following stroke may impact aorta vascular reactivity.
  • Targeting inflammatory cytokines could potentially alleviate stroke-associated peripheral vascular dysfunction.