True lab-in-a-syringe technology for bioassays.

Area of Science:

• Analytical Chemistry
• Materials Science

Background:

• Traditional Lab-in-a-syringe (LIS) tests face challenges in integrated reaction and detection.
• Enhancing result visibility and simplifying user interaction is crucial for point-of-care diagnostics.

Purpose of the Study:

• To introduce a novel lab-in-a-syringe test design integrating reaction and detection pads.
• To improve the visibility and user-friendliness of LIS tests.
• To demonstrate the concept's efficacy for specific ion determination, such as Nickel(II).

Main Methods:

• Developed a lab-in-a-syringe system with integrated reaction and detection pads within the syringe barrel.
• Investigated two detection pad layouts: rounded and rectangular.
• Utilized dimethylglyoxime for Nickel(II) determination and Fe(II) interference blocking.

Main Results:

• Achieved excellent calibration for Ni(II) determination with R² of 0.998 under optimal conditions.
• Demonstrated effective blocking of Fe(II) interference.
• Estimated production costs at an economical 0.2 USD per test.

Conclusions:

• The novel lab-in-a-syringe design offers a viable and cost-effective approach for chemical analysis.
• This integrated system enhances user experience and result visibility for LIS tests.
• The proof-of-concept successfully determined Ni(II) with high accuracy and minimal interference.