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Quantification of intercellular adhesion forces measured by fluid force microscopy.

Noa Cohen1, Saheli Sarkar1, Evangelia Hondroulis1

  • 1Department of Pharmaceutical Sciences, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, United States.

Talanta
|July 26, 2017
PubMed
Summary

Fluidic force microscopy (FluidFM) effectively measures cancer cell adhesion forces. Adhesion trends differed between homotypic and heterotypic cell pairs over time, revealing insights into tumor progression.

Keywords:
AdhesionBreast cancerFluidic force microscopy (FluidFM)

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Area of Science:

  • Cell Biology
  • Biophysics
  • Cancer Research

Background:

  • Intercellular adhesion is crucial for tumor progression and metastasis.
  • Previous methods for quantifying cell adhesion include atomic force microscopy.
  • Understanding cancer cell adhesion mechanics is vital for developing new therapies.

Purpose of the Study:

  • To demonstrate the feasibility of fluidic force microscopy (FluidFM) for measuring breast cancer cell adhesion forces.
  • To compare homotypic and heterotypic cancer cell adhesion dynamics.
  • To assess the impact of contact duration on cellular adhesion.

Main Methods:

  • Utilized fluidic force microscopy (FluidFM) to measure adhesive forces between cell pairs.
  • Employed pressure-dependent immobilization of cells at the probe tip for controlled contact.
  • Assessed adhesion forces at varying contact durations (short < 1min and long 30min).
  • Avoided chemical fixation to maintain cell physiology and allow probe reuse.

Main Results:

  • FluidFM successfully measured adhesive forces exerted by breast cancer cells.
  • Adhesion forces were initially similar for homotypic (cancer-cancer) and heterotypic (cancer-fibroblast, cancer-epithelial) cell pairs.
  • Distinct trends in adhesion forces emerged between cell types at longer contact durations (30min).

Conclusions:

  • FluidFM is a rapid and efficient technique for assessing cellular adhesion.
  • The method can reveal heterogeneity in cell adhesion relevant to different stages of cancer.
  • Findings highlight the dynamic nature of cancer cell interactions and their potential role in metastasis.