Related Experiment Video
Updated: Feb 26, 2026
07:23
Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
Published on: May 30, 2025
1.2K
Oncogenic long noncoding RNA landscape in breast cancer
Shouping Xu1, Dejia Kong1, Qianlin Chen1
1Department of Breast Surgery, Harbin Medical University Cancer Hospital, 150 Haping Road, Harbin, 150081, China.
Molecular Cancer
|July 26, 2017
Summary
This study identified novel oncogenic long noncoding RNAs (lncRNAs) in breast cancer, revealing their role in tumor progression and potential as therapeutic targets. Understanding these lncRNAs improves breast cancer management.
Area of Science:
- Oncology
- Molecular Biology
- Genomics
Background:
- Limited identification of long noncoding RNAs (lncRNAs) acting as oncogenes in breast cancer.
- Oncogenic lncRNAs are linked to tumor development and poorer survival outcomes.
Purpose of the Study:
- To identify and validate oncogenic lncRNAs in breast cancer.
- To investigate their biological functions, regulatory mechanisms, and subtype-specific roles.
- To elucidate the mechanisms driving lncRNA upregulation and validate their oncogenic functions in vitro.
Main Methods:
- Utilized Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) for lncRNA validation.
- Employed bioinformatics, genome-wide data analysis, and high-throughput sequencing.
- Performed in vitro assays including proliferation, flow cytometry, and TUNEL assays.
Main Results:
- Identified upregulated oncogenic lncRNAs associated with worse survival in breast cancer.
- Discovered lncRNAs regulate immune activation and TGF-beta/Jak-STAT pathways.
- Found TINCR, LINC00511, and PPP1R26-AS1 are subtype-specific; gene amplification drives upregulation.
Conclusions:
- Expanded the knowledge of oncogenic lncRNAs in breast cancer.
- Provided insights into their regulatory mechanisms and functional roles.
- Findings may contribute to improved breast cancer management strategies.
Related Concept Videos
02:39lncRNA - Long Non-coding RNAs
10.0K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
10.0K
02:39lncRNA - Long Non-coding RNAs
3.7K
3.7K
03:18Non-LTR Retrotransposons
13.5K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
13.5K
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
9.9K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
9.9K
01:22MicroRNAs
4.2K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
01:33Cancer-Critical Genes I: Proto-oncogenes
11.6K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
11.6K