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Updated: Feb 26, 2026

Amplicon Sequencing using the Long-Read Sequencing Technologies
Published on: August 29, 2025
Next-generation sequencing to monitor the spread of antimicrobial resistance
1Pathogen Molecular Genetics Section, Laboratory of Bacteriology, National Institute of Allergy and Infectious Diseases, U.S. National Institutes of Health, Bethesda, MD, 20814, USA. motto@niaid.nih.gov.
Abstract:
Next-generation sequencing is increasingly being used to monitor current and historic events related to the emergence and spread of antimicrobial resistance. In a recent publication, researchers analyzed the rise of methicillin-resistant Staphylococcus aureus in the 1960s, emphasizing that adaptations conferring antibiotic resistance might pre-date the introduction of novel antibiotic derivatives. Other researchers have evaluated the role of transmission within a healthcare network, using the example of extended-spectrum beta-lactamase-resistant Escherichia coli.Please see related Genome Biology Research article: www.dx.doi.org/10.1186/s13059-017-1252-9 and Genome Medicine Research article: www.dx.doi.org/10.1186/s13073-017-0457-6.
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