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Key differences between apoC-III regulation and expression in intestine and liver
Gabrielle West1, Cayla Rodia1, Diana Li1
1Department of Nutritional Sciences, University of Connecticut, 1392 Storrs Rd, Storrs, CT 06269-4017, United States.
Biochemical and Biophysical Research Communications
|July 26, 2017
Summary
Intestinal apolipoprotein C-III (ApoC-III) is not regulated by common nutrients or FoxO1, unlike its liver counterpart. This finding highlights unique intestinal regulation of ApoC-III, a key cardiovascular risk factor.
Area of Science:
- Biochemistry
- Molecular Biology
- Cardiovascular Science
Background:
- Apolipoprotein C-III (ApoC-III) is a key factor in cardiovascular disease (CVD) risk.
- Hepatic (liver) regulation of ApoC-III is well-studied due to its role in plasma lipids.
- Intestinal regulation of ApoC-III remains largely unknown.
Purpose of the Study:
- To investigate the regulatory mechanisms of intestinal ApoC-III expression.
- To compare intestinal ApoC-III regulation with hepatic ApoC-III regulation.
- To determine if FoxO1 influences intestinal ApoC-III expression.
Main Methods:
- Primary murine enteroid cultures.
- Caco-2 cell line studies.
- Dietary intervention studies in wild-type mice.
Main Results:
- Intestinal ApoC-III expression is unresponsive to fatty acids, glucose, or insulin.
- Intestinal ApoC-III is not affected by changes in FoxO1 expression.
- Western diet does not alter intestinal ApoC-III, contrasting with hepatic responses.
Conclusions:
- Intestinal ApoC-III is not a FoxO1 target.
- Intestinal ApoC-III is regulated distinctly from hepatic ApoC-III.
- These findings reveal unique characteristics of ApoC-III regulation in the intestine.
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