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Reduced Expression of Metastasis Suppressor-1 (MTSS1) Accelerates Progression of Human Bladder Uroepithelium Cell
Peng Du1, Shuo Wang1, Xingxing Tang1
1Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Urology, Peking University Cancer Hospital & Institute, Beijing, P.R. China.
Background:
Metastasis suppressor 1 (MTSS1) is a multi-functional cytoskeletal protein. Recent research showed that MTSS1 is a potential tumor suppressor in many types of cancer cells, including kidney and bladder cancer cells. However, the clinical implication of MTSS1 in human bladder uroepithelium cell carcinoma (BUCC) and its potential in suppressing BUCC tumorigenesis remains undetermined. In the present study, the expression of MTSS1 in human BUCC tissue samples, and correlations between MTSS1 and pathological grade and stage of the tumors were examined in BUCC specimens. The function of MTSS1 in BUCC progression was explored.
Materials And Methods:
The mRNA and protein expression of MTSS1 were examined in 68 BUCC tissue samples with matching adjacent normal bladder tissues using quantitative real-time PCR and western blotting. Furthermore, the bladder cancer cell line 5637 was used to determine the anticancer effect of MTSS1.
Results:
Lower MTSS1 mRNA expression was recorded in BUCC tissues compared to normal bladder tissues. A lower MTSS1 mRNA level was observed in tumors with high clinical stage and with high pathological nuclear grade. Likewise, MTSS1 protein expression in normal bladder tissue was significantly higher than that in BUCC tissue. The protein level of MTSS1 significantly negatively correlated with clinical stage and pathological nuclear grade of BUCC. Cumulative survival curves indicated that MTSS1 expression was negatively correlated with survival time: patients with a high level of MTSS1 had significantly longer survival time than those with a low level of MTSS1 (p<0.001). Overexpression of MTSS1 reduced BUCC cell proliferation, cell-cycle progression and colony formation, but had no influence on BUCC cell apoptosis.
Conclusion:
Overexpression of MTSS1 suppresses BUCC development, providing a novel perspective for BUCC tumorigenesis and a potential therapeutic target for BUCC.
Insights
Metastasis suppressor 1 (MTSS1) is downregulated in bladder cancer. Higher MTSS1 levels correlate with better patient survival and suppressed tumor growth, suggesting MTSS1 as a potential therapeutic target for bladder uroepithelium cell carcinoma (BUCC).
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Metastasis suppressor 1 (MTSS1) is a cytoskeletal protein with potential tumor suppressor functions.
- Previous studies indicated MTSS1's role in kidney and bladder cancers, but its clinical significance in bladder uroepithelium cell carcinoma (BUCC) was unclear.
Purpose of the Study:
- To investigate the clinical implication of MTSS1 in human BUCC.
- To explore the function of MTSS1 in suppressing BUCC tumorigenesis and progression.
Main Methods:
- Examined MTSS1 mRNA and protein expression in 68 BUCC tissues and adjacent normal tissues using qRT-PCR and Western blotting.
- Assessed the correlation between MTSS1 expression and clinicopathological features (grade, stage).
- Investigated the effect of MTSS1 overexpression on BUCC cell line 5637 proliferation, cell cycle, colony formation, and apoptosis.
Main Results:
- MTSS1 mRNA and protein levels were significantly lower in BUCC tissues compared to normal tissues.
- Reduced MTSS1 expression correlated with higher clinical stage and pathological nuclear grade.
- High MTSS1 expression was associated with significantly longer patient survival (p<0.001).
- Overexpression of MTSS1 inhibited BUCC cell proliferation, cell-cycle progression, and colony formation without affecting apoptosis.
Conclusions:
- MTSS1 functions as a tumor suppressor in BUCC.
- MTSS1 expression levels are a prognostic indicator for BUCC patients.
- MTSS1 represents a potential therapeutic target for bladder uroepithelium cell carcinoma.
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