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Reduced expression of E-cadherin and increased sialylation level in clear cell renal cell carcinoma

Małgorzata Borzym-Kluczyk1, Iwona Radziejewska2, Marzanna Cechowska-Pasko1

  • 1Department of Pharmaceutical Biochemistry, Medical University of Bialystok, Białystok, Poland.

Acta Biochimica Polonica
|July 26, 2017
PubMed

Insights

Clear cell renal cell carcinoma (RCC) shows decreased E-cadherin expression and increased sialylated oligosaccharides. These changes may serve as non-invasive biomarkers for early kidney cancer detection.

Area of Science:

  • Oncology
  • Glycobiology
  • Biochemistry

Background:

  • Cancer cells exhibit increased N-glycosylation and disrupted E-cadherin-mediated adherens junctions.
  • The link between altered N-glycosylation and E-cadherin loss in cancer is not fully understood.
  • Mechanisms of altered adhesive glycoprotein expression in cancer cells require further elucidation.

Purpose of the Study:

  • To investigate E-cadherin expression and specific sialylated structures (sialyl Lewis a/x, NeuAcα2-3Gal, NeuAcα2-6Gal/GalNAc) in normal, intermediate, and cancerous renal tissues of clear cell RCC patients.
  • To correlate E-cadherin expression with specific sugar residues in renal cancer tissue glycoproteins.
  • To explore potential non-invasive biomarkers for early RCC detection.

Main Methods:

  • E-cadherin expression was analyzed using ELISA and immunoblotting.
  • Oligosaccharide structures and sialylation levels were detected via ELISA with specific lectins and antibodies.
  • Analysis was performed on normal renal tissue, intermediate zone tissue, and cancerous tissue from clear cell RCC patients.

Main Results:

  • A significant decrease in E-cadherin expression was observed in intermediate and cancerous renal tissues compared to normal tissue.
  • A significant increase in sialylated oligosaccharide levels was found in intermediate and cancerous renal tissues versus normal tissue.
  • Renal cancer tissue showed significantly lower cadherin expression and higher oligosaccharide sialylation compared to both normal and intermediate zone tissues.

Conclusions:

  • Decreased E-cadherin expression and increased sialylation of oligosaccharide structures are characteristic of renal cancer.
  • These alterations present a potential avenue for developing non-invasive biomarkers for early RCC detection.
  • Quantification of cadherins and sialylated oligosaccharides in urine or urinary sediment warrants further investigation for early RCC diagnosis.
Keywords:
E-cadherinclear cell renal cell carcinomalectinssialyl Lewis antigens

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