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A surface plasmon field-enhanced fluorescence reversible split aptamer biosensor.

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  • 1BioSensor Technologies, AIT-Austrian Institute of Technology, Muthgasse 11, 1190 Vienna, Austria. jakub.dostalek@ait.ac.at.

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Summary
This summary is machine-generated.

This study presents a biosensor using surface plasmon field-enhanced fluorescence for detecting analytes with low affinity split aptamer ligands. This method amplifies weak signals, enabling sensitive and specific real-time monitoring in complex samples.

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Area of Science:

  • Biosensing
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Weak affinity ligands offer advantages for continuous monitoring biosensors due to their reversible binding.
  • Traditional assays with high affinity ligands require regeneration steps, limiting real-time analysis.
  • Low affinity ligands in fluorescence sensors face challenges with weak optical signals and high background noise.

Purpose of the Study:

  • To develop a biosensor utilizing surface plasmon field-enhanced fluorescence for sensitive detection of analytes.
  • To overcome the limitations of weak optical responses and background noise associated with low affinity ligands.
  • To demonstrate real-time monitoring capabilities for analytes like adenosine and adenosine triphosphate.

Main Methods:

  • Utilized heterogeneous assays with low affinity split aptamer ligands.
  • Coupled fluorophore labels with confined surface plasmon fields for signal amplification.
  • Employed time-resolved analysis for monitoring target analytes.

Main Results:

  • Achieved strong amplification of fluorescence signals emitted from the sensor surface.
  • Demonstrated efficient discrimination of the specific signal from background noise.
  • Successfully performed time-resolved analysis of adenosine and adenosine triphosphate with a split aptamer (Kd 0.73–1.35 mM).

Conclusions:

  • The developed biosensor enables rapid and specific discrimination of target analyte concentration changes.
  • The surface plasmon field-enhanced fluorescence approach overcomes signal limitations of low affinity ligands.
  • The biosensor is effective for detecting analytes in both buffer and serum samples across a wide concentration range (μM to mM).