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Analysis of root surface properties by fluorescence/Raman intensity ratio
Shino Nakamura1, Masahiro Ando1, Hiro-O Hamaguchi1
1Department of Periodontology, Showa University School of Dentistry, 2-1-1, Kitasenzoku, Ohta-ku, Tokyo, 145-8515, Japan.
Lasers in Medical Science
|July 27, 2017
Summary
This study shows that the fluorescence/Raman intensity ratio can detect residual calculus on tooth root surfaces. This method effectively indicates when calculus removal is complete, aiding dental procedures.
Area of Science:
- Biomedical Engineering
- Dental Spectroscopy
- Oral Health Diagnostics
Background:
- Residual calculus on root surfaces is a significant challenge in periodontal therapy.
- Accurate detection of calculus is crucial for effective debridement and treatment outcomes.
- Current methods for calculus detection can be subjective and may miss small deposits.
Purpose of the Study:
- To evaluate the fluorescence/Raman intensity ratio as a method for detecting residual calculus on human root surfaces.
- To assess the efficacy of this ratio in confirming complete calculus removal after debridement.
Main Methods:
- Utilized a portable Raman spectrophotometer with a 785-nm laser on 32 extracted human teeth with calculus.
- Collected Raman spectra from calculus, cementum, and dentin, normalizing to the hydroxyapatite band at 960 cm⁻¹.
- Measured the fluorescence/Raman intensity ratio before and after three debridement strokes on 25 teeth.
Main Results:
- Raman spectra differed significantly between calculus, cementum, and dentin.
- The fluorescence/Raman intensity ratio was significantly higher for calculus compared to root surfaces (p < 0.05).
- This ratio decreased with debridement, approaching baseline values as calculus was removed, indicating successful debridement.
Conclusions:
- The fluorescence/Raman intensity ratio is a sensitive and objective tool for detecting residual calculus.
- This spectroscopic approach can effectively confirm the completion of root surface debridement.
- The method offers potential for improved diagnostic accuracy in periodontal therapy.
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