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Published on: July 26, 2024
Inheritance and Bulked Segregant Analysis of Leaf Rust and Stem Rust Resistance in Durum Wheat Genotypes
Meriem Aoun1, James A Kolmer1, Matthew N Rouse1
1First author: Department of Plant Pathology, North Dakota State University, Fargo 58108; second and third authors: United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Cereal Disease Laboratory, and Department of Plant Pathology, University of Minnesota, St. Paul 55108; fourth author: USDA-ARS Genotyping Laboratory, Biosciences Research Laboratory, Fargo, ND, 58102; fifth author: Ethiopian Institute of Agricultural Research, P.O. Box 32, Debre Zeit, Ethiopia; sixth author: Department of Plant Sciences, North Dakota State University; and seventh author: International Programs, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY.
Abstract:
Leaf rust, caused by Puccinia triticina, and stem rust, caused by P. graminis f. sp. tritici, are important diseases of durum wheat. This study determined the inheritance and genomic locations of leaf rust resistance (Lr) genes to P. triticina race BBBQJ and stem rust resistance (Sr) genes to P. graminis f. sp. tritici race TTKSK in durum accessions. Eight leaf-rust-resistant genotypes were used to develop biparental populations. Accessions PI 192051 and PI 534304 were also resistant to P. graminis f. sp. tritici race TTKSK. The resulting progenies were phenotyped for leaf rust and stem rust response at seedling stage. The Lr and Sr genes were mapped in five populations using single-nucleotide polymorphisms and bulked segregant analysis. Five leaf-rust-resistant genotypes carried single dominant Lr genes whereas, in the remaining accessions, there was deviation from the expected segregation ratio of a single dominant Lr gene. Seven genotypes carried Lr genes different from those previously characterized in durum. The single dominant Lr genes in PI 209274, PI 244061, PI387263, and PI 313096 were mapped to chromosome arms 6BS, 2BS, 6BL, and 6BS, respectively. The Sr gene in PI 534304 mapped to 6AL and is most likely Sr13, while the Sr gene in PI 192051 could be uncharacterized in durum.
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