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Related Experiment Video

Updated: Feb 25, 2026

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization
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Sortase-Mediated Surface Functionalization of Stimuli-Responsive Microgels.

Elisabeth Gau1,2, Diana M Mate2, Zhi Zou2,3

  • 1Functional and Interactive Polymers, Institute of Technical and Macromolecular Chemistry, RWTH Aachen University , Worringerweg 2, 52074, Aachen, Germany.

Biomacromolecules
|July 27, 2017
PubMed
Summary

This study presents an enzyme-mediated method for attaching biomolecules to microgels. Sortase-mediated coupling allows precise surface functionalization of stimuli-responsive microgels with proteins like eGFP.

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Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Bioconjugation Chemistry

Background:

  • Stimuli-responsive microgels offer tunable properties for various applications.
  • Surface functionalization is crucial for integrating biomolecules with microgel platforms.
  • Enzyme-mediated strategies provide high specificity for bioconjugation.

Purpose of the Study:

  • To develop an enzyme-mediated method for selective biomolecule decoration of aqueous microgels.
  • To functionalize poly(N-vinylcaprolactam) (VCL) microgels with a specific peptide sequence.
  • To conjugate enhanced Green Fluorescent Protein (eGFP) to the microgel surface using Sortase A.

Main Methods:

  • Synthesis and characterization of poly(N-vinylcaprolactam)/glycidyl methacrylate (VCL/GMA) microgels.
  • Surface modification of microgels by grafting LPETG peptide for Sortase A recognition.
  • Sortase-mediated conjugation of eGFP to the modified microgel surface.

Main Results:

  • Successful synthesis and characterization of VCL/GMA microgels with tunable properties.
  • Demonstrated efficient and specific conjugation of eGFP to the microgel surface via Sortase A.
  • Quantified precise control over the amount of eGFP grafted, evidenced by fluorescence intensity measurements.

Conclusions:

  • Sortase-mediated coupling is a powerful and simple technique for targeted surface functionalization of stimuli-responsive microgels.
  • This method allows for controlled attachment of biomolecules, enhancing microgel utility.
  • The developed protocol offers precise control over biomolecule grafting for advanced applications.