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Published on: May 17, 2016
Osteopontin is linked with AKT, FoxO1, and myostatin in skeletal muscle cells
Peter P Nghiem1, Joe N Kornegay1, Kitipong Uaesoontrachoon2
1Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, 4458 TAMU, Texas A&M University, College Station, Texas, 77843-4458, USA.
Introduction:
Osteopontin (OPN) polymorphisms are associated with muscle size and modify disease progression in Duchenne muscular dystrophy (DMD). We hypothesized that OPN may share a molecular network with myostatin (MSTN).
Methods:
Studies were conducted in the golden retriever (GRMD) and mdx mouse models of DMD. Follow-up in-vitro studies were employed in myogenic cells and the mdx mouse treated with recombinant mouse (rm) or human (Hu) OPN protein.
Results:
OPN was increased and MSTN was decreased and levels correlated inversely in GRMD hypertrophied muscle. RM-OPN treatment led to induced AKT1 and FoxO1 phosphorylation, microRNA-486 modulation, and decreased MSTN. An AKT1 inhibitor blocked these effects, whereas an RGD-mutant OPN protein and an RGDS blocking peptide showed similar effects to the AKT inhibitor. RMOPN induced myotube hypertrophy and minimal Feret diameter in mdx muscle.
Discussion:
OPN may interact with AKT1/MSTN/FoxO1 to modify normal and dystrophic muscle. Muscle Nerve 56: 1119-1127, 2017.
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