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MicroRNA-143 inhibits cell growth by targeting ERK5 and MAP3K7 in breast cancer

L L Zhou1, J L Dong2, G Huang2

  • 1Department of Cardiology, Huaihe Hospital of Henan University, Kaifeng, China.

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas
|July 27, 2017
PubMed

Insights

MicroRNA-143 (miR-143) is decreased in breast cancer (BC) tissues. Upregulating miR-143 inhibits BC cell growth by targeting extracellular signal-regulated kinase 5 (ERK5) and mitogen-activated protein 3 kinase 7 (MAP3K7).

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • MicroRNA-143 (miR-143) plays a role in cancer development.
  • The specific function and mechanism of miR-143 in breast cancer (BC) require further investigation.
  • Extracellular signal-regulated kinase 5 (ERK5) and mitogen-activated protein 3 kinase 7 (MAP3K7) are implicated in cellular processes.

Purpose of the Study:

  • To investigate the role and mechanism of miR-143 in the occurrence and development of breast cancer (BC).
  • To determine the relationship between miR-143, ERK5, and MAP3K7 in BC tissues.
  • To evaluate the effect of miR-143 modulation on BC cell proliferation and signaling pathways.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR), western blotting, and immunohistochemistry were used to detect miR-143, ERK5, and MAP3K7 levels in BC and normal tissues.
  • Pearson correlation analysis assessed the association between miR-143 and ERK5/MAP3K7.
  • MCF-7 cells were transfected with miR-143 mimic/inhibitor and siRNAs targeting ERK5, MAP3K7, or cyclin D1 to assess cell viability and protein expression.

Main Results:

  • miR-143 levels were significantly decreased in BC tissues compared to noncancerous and normal tissues.
  • Expressions of p-ERK5, ERK5, p-MAP3K7, and MAP3K7 were elevated in BC tissues and negatively correlated with miR-143 levels.
  • Upregulation of miR-143 inhibited MCF-7 cell viability and decreased the expression of p-ERK5, ERK5, p-MAP3K7, MAP3K7, and cyclin D1.

Conclusions:

  • ERK5 and MAP3K7 are identified as potential target genes of miR-143.
  • Increased miR-143 expression suppresses breast cancer cell growth, potentially through the regulation of ERK5 and MAP3K7 signaling pathways.
  • miR-143 may serve as a tumor suppressor in breast cancer development.

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