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Updated: Feb 25, 2026

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
Published on: July 25, 2022
Imaging chemistry inside living cells by stimulated Raman scattering microscopy
Hyeon Jeong Lee1, Ji-Xin Cheng2
1Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA; Interdisciplinary Life Science Program, Purdue University, West Lafayette, IN 47907, USA.
Abstract:
Stimulated Raman scattering (SRS) microscopy is a vibrational imaging platform developed to visualize chemical content of a biological sample based on molecular vibrational fingerprints. With high-speed, high-sensitivity, and three-dimensional sectioning capability, SRS microscopy has been used to study chemical distribution, molecular transport, and metabolic conversion in living cells in a label-free manner. Moreover, aided with bio-orthogonal small-volume Raman probes, SRS microscopy allows direct imaging of metabolic activities of small molecules in living cells.
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