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Mitotic nuclear pore complex segregation involves Nup2 in Aspergillus nidulans
Subbulakshmi Suresh1,2, Sarine Markossian1, Aysha H Osmani1
1Department of Molecular Genetics, The Ohio State University, Columbus, OH.
The Journal of Cell Biology
|July 28, 2017
Summary
The nucleoporin Nup2 is essential for distributing nuclear pore complexes (NPCs) to daughter cells during mitosis in Aspergillus nidulans. Its chromatin association during mitosis links DNA and NPC segregation.
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- Nuclear pore complexes (NPCs) regulate transport between the nucleus and cytoplasm during interphase.
- The nucleoporin Nup2 facilitates interphase transport via importin α- and Ran-binding domains.
- Nup2 also localizes to chromatin during mitosis in Aspergillus nidulans and vertebrates, suggesting mitotic roles.
Purpose of the Study:
- To investigate the role of Nup2 in mitotic nuclear pore complex (NPC) inheritance in Aspergillus nidulans.
- To determine the functional domains of Nup2 required for its mitotic function.
- To elucidate the mechanism by which Nup2 links NPC segregation with DNA segregation during mitosis.
Main Methods:
- Genetic analysis in Aspergillus nidulans.
- Localization studies of Nup2 during mitosis.
- Functional domain analysis of Nup2.
- Experimental manipulation to create an artificial link between NPCs and chromatin.
Main Results:
- Nup2 is essential for mitotic NPC inheritance in Aspergillus nidulans.
- The mitotic function of Nup2 in NPC segregation is independent of its importin α- and Ran-binding domains.
- A central targeting domain of Nup2 is crucial for its localization and viability during mitosis.
- Bypassing the requirement for Nup2 by artificially linking NPCs and chromatin confirmed its role in bridging NPC and DNA segregation.
Conclusions:
- Nup2 plays a critical role in ensuring accurate mitotic NPC segregation to daughter nuclei.
- Mitotic chromatin association of Nup2 acts as a crucial link between DNA and NPC segregation.
- The findings reveal a novel mechanism for coordinating nuclear and chromosomal segregation during cell division.
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