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Chlorophyll Content Assay to Quantify the Level of Necrosis Induced by Different R Gene/Elicitor Combinations after
C Jake Harris1, David C Baulcombe1
1Plant Sciences Department, Cambridge University, Cambridge, UK.
Bio-Protocol
|July 28, 2017
Abstract:
This assay can be used to rapidly and accurately quantify levels of leaf necrosis induced after transient expression of R genes and elicitor combinations (Harris et al., 2013). It is based on the inverse correlation between level of necrosis and chlorophyll content in leaf tissue. It is adapted from the calculations described by (Strain et al., 1971).
