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Related Concept Videos

Applications Of NMR In Biology01:25

Applications Of NMR In Biology

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Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts
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NMR and MS Methods for Metabolomics.

Alexander Amberg1, Björn Riefke2, Götz Schlotterbeck3

  • 1Preclinical Safety, Sanofi R&D, Hattersheim, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|July 28, 2017
PubMed
Summary
This summary is machine-generated.

Metabolomics, the study of metabolites, offers direct phenotype insights, driving its rise in drug development and molecular medicine. Advanced technologies like NMR and UPLC-MS enable comprehensive metabolic profiling for better understanding of biological changes.

Keywords:
GC-MSMetabolic profilingMetabolomicsNMRUPLC-MS

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Area of Science:

  • Biotechnology
  • Molecular Medicine
  • Pharmacology

Background:

  • Metabolomics, or metabolic profiling, systematically analyzes metabolites in biological samples.
  • It offers direct phenotype insights, distinguishing it from other omics technologies.
  • Its popularity has surged due to advancements in analytical and bioinformatics tools.

Purpose of the Study:

  • To detail the best practices in metabolomics for drug development and molecular medicine.
  • To provide a comprehensive overview of the entire metabolic profiling workflow.
  • To guide researchers in applying metabolomics for phenotype analysis.

Main Methods:

  • Utilizes advanced analytical platforms such as Nuclear Magnetic Resonance (NMR), Ultra-Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS), and Gas Chromatography-Mass Spectrometry (GC-MS).
  • Employs systematic sample preparation techniques tailored for metabolite extraction and stability.
  • Incorporates detailed data analysis strategies specific to metabolomics.

Main Results:

  • Sensitive and highly reproducible analytical platforms allow for parallel determination of hundreds of metabolites.
  • Detailed methodologies cover all critical steps from sample preparation to data interpretation.
  • Best practices are established for robust and reliable metabolic profiling.

Conclusions:

  • Metabolomics is a powerful tool for understanding biological phenotypes and their alterations.
  • Standardized best practices are crucial for successful application in drug development and molecular medicine.
  • Technological advancements continue to enhance the scope and precision of metabolic profiling.