Electrical properties of a Na+-dependent phenylalanine transport in lizard (Lacerta galloti) duodenum

The unidirectional transepithelial fluxes of L-phenylalanine across lizard duodenum were determined in flux chambers. Phenylalanine was preferentially transferred from the mucosal to the serosal fluid. This transport was accompanied by an accumulation of substrate from the mucosal medium into the tissue to a similar level and against a concentration gradient. There was no net movement of phenylalanine when the sodium was substituted by choline. The influx of L-phenylalanine into the epithelial cells of lizard duodenum was examined by incubating slices of intestine in radioactively-labelled solutions of the substrate for 2 min. The steady-state uptake was assessed after similar incubations lasting 45 min. Phenylalanine influx obeys the Michaelis-Menten equation with a Km of 5.1 and is dependent on the presence of sodium ions in the incubation medium. Phenylalanine has been used to induce changes in short-circuit current (delta Isc) across intestine. delta Isc was a hyperbolic function of amino acid concentration characterized by the parameters Jm (maximum change in delta Isc) and Km (concentration needed to attain an delta Isc equal to half the Jm). delta Isc determined Km constants showed good agreement with values obtained from direct measurements of phenylalanine uptake into tissue.