Related Experiment Video
Updated: Feb 25, 2026
10:47
Harnessing the Bioorthogonal Inverse Electron Demand Diels-Alder Cycloaddition for Pretargeted PET Imaging
Published on: February 3, 2015
9.3K
Alternative reagents to antibodies in imaging applications
R Bedford1, C Tiede1, R Hughes1
1School of Molecular and Cellular Biology, Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, UK.
Biophysical Reviews
|July 29, 2017
Summary
Novel non-antibody binding reagents offer specific, reproducible alternatives to traditional antibodies for molecular biology and research. These reagents, including Affimers, show promise in various applications like imaging.
Area of Science:
- Biochemistry
- Molecular Biology
- Medical Research
Background:
- Antibodies are essential research tools but face challenges in validation, specificity, and batch consistency.
- These limitations drive the development of alternative non-antibody binding reagents.
Purpose of the Study:
- To review the success of non-antibody binding reagents in imaging applications.
- To highlight novel reagents such as Affimers as alternatives to antibodies.
Main Methods:
- Review of existing literature on non-antibody binding reagents.
- Analysis of applications in molecular imaging.
Main Results:
- Non-antibody reagents demonstrate high specificity and ease of production.
- Site-direct labeling capabilities enable diverse applications.
- Affimers are emerging as successful non-antibody reagents in imaging.
Conclusions:
- Non-antibody binding reagents provide reliable and specific alternatives to antibodies.
- These reagents offer advantages in production, labeling, and application diversity, particularly in imaging.
