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Published on: May 18, 2022
Design, Cloning, and In Vitro Screening of Artificial miRNAs to Silence Alpha-1 Antitrypsin
Florie Borel1, Christian Mueller2,3
1Horae Gene Therapy Center, University of Massachusetts Medical School, 55 Lake Ave. North, Worcester, MA, 01605, USA. florie.borel@umassmed.edu.
Abstract:
This protocol describes the design, cloning, and in vitro screening of artificial microRNAs (miRNAs) to silence alpha-1 antitrypsin (AAT). This method would be of interest to silence AAT in a variety of in vitro or in vivo models, and prevalidated sequences against human AAT are provided. This simple 5-day protocol may more generally be used to design artificial miRNAs against any transcript.
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