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Related Experiment Video

Updated: Feb 25, 2026

Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas
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Immunohistochemistry Staining for Human Alpha-1 Antitrypsin.

Dongtao A Fu1, Martha Campbell-Thompson2

  • 1Department of Pathology, Immunology, and Laboratory Medicine, College of Medicine, University of Florida, 1395 Center Drive, Gainesville, FL, 32610, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 29, 2017
PubMed
Summary

This study presents a highly sensitive immunohistochemistry (IHC) method for detecting Alpha-1 Antitrypsin (AAT) protein. This advancement improves the detection of AAT in both human and mouse tissues for research and gene therapy applications.

Keywords:
Alpha-1 antitrypsinAlpha-1 antitrypsin deficiencyImmunohistochemistryImmunoperoxidaseProtein localization

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Area of Science:

  • Immunology
  • Molecular Biology
  • Histology

Background:

  • Immunohistochemistry (IHC) is a key technique for visualizing protein localization in tissues.
  • Advancements in IHC enhance sensitivity and specificity for novel discoveries.
  • Alpha-1 Antitrypsin (AAT) IHC is crucial for studying AAT expression and gene therapy efficacy.

Purpose of the Study:

  • To describe a novel, highly sensitive method for detecting the AAT antigen.
  • To enable precise analysis of AAT protein expression in various tissue types.

Main Methods:

  • Development of a sensitive immunohistochemistry protocol.
  • Application to formalin-fixed paraffin-embedded human and mouse tissues.
  • Detection of the Alpha-1 Antitrypsin antigen.

Main Results:

  • A highly sensitive method for AAT antigen detection was successfully established.
  • The method is applicable to both human and mouse formalin-fixed paraffin-embedded tissues.
  • Demonstrated improved sensitivity for AAT protein analysis.

Conclusions:

  • The developed IHC method offers enhanced sensitivity for AAT detection.
  • This technique supports research in liver diseases and AAT gene therapy.
  • Provides a valuable tool for precise protein localization studies.