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Updated: Feb 25, 2026
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Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining
Published on: December 23, 2014
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Periodic Acid-Schiff Staining with Diastase
Dongtao A Fu1, Martha Campbell-Thompson2
1Department of Pathology, Immunology, and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL, 32610, USA.
Methods in Molecular Biology (Clifton, N.J.)
|July 29, 2017
Summary
Periodic Acid-Schiff with diastase (PAS-D) staining differentiates glycogen from other elements in tissue. This method aids in diagnosing alpha-1 antitrypsin deficiency by highlighting liver AAT polymer inclusions.
Area of Science:
- Histopathology
- Biochemical Staining Techniques
Background:
- Periodic Acid-Schiff (PAS) staining identifies carbohydrates like glycogen.
- Diastase is an enzyme that specifically digests glycogen.
- Distinguishing glycogen from other PAS-positive substances is crucial in tissue analysis.
Purpose of the Study:
- To detail the procedure for Periodic Acid-Schiff with diastase (PAS-D) staining.
- To demonstrate the utility of PAS-D in differentiating glycogen in human liver tissues.
- To highlight PAS-D's application in identifying alpha-1 antitrypsin deficiency.
Main Methods:
- Utilizing PAS staining in conjunction with diastase enzyme digestion.
- Applying the PAS-D procedure to formalin-fixed, paraffin-embedded human liver tissues.
- Microscopic examination of stained tissue sections for characteristic inclusions.
Main Results:
- Successful differentiation of glycogen from other PAS-positive materials in liver samples.
- Visualization of periportal AAT polymer inclusions in alpha-1 antitrypsin deficiency.
- Demonstration of the specificity of diastase in glycogen digestion.
Conclusions:
- The PAS-D staining method is effective for glycogen differentiation in histopathology.
- PAS-D staining is a valuable tool for diagnosing alpha-1 antitrypsin deficiency.
- Standardized PAS-D staining protocols are essential for accurate diagnostic interpretation.
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