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Updated: Feb 25, 2026

Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins
Published on: December 12, 2013
Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins
Vilius Kurauskas1,2,3, Paul Schanda1,2,3, Remy Sounier4
1IBS, Université Grenoble Alpes, F-38044, Grenoble, France.
Abstract:
Methyl groups are very useful probes of structure, dynamics, and interactions in protein NMR spectroscopy. In particular, methyl-directed experiments provide high sensitivity even in very large proteins, such as membrane proteins in a membrane-mimicking environment. In this chapter, we discuss the approach for labeling methyl groups in E. coli-based protein expression, as exemplified with the mitochondrial carrier GGC.
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