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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Digital Microfluidics for Automated Proteomic Processing
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Microfluidic-Mass Spectrometry Interfaces for Translational Proteomics.

R Daniel Pedde1, Huiyan Li2, Christoph H Borchers3

  • 1Laboratory for Innovations in Microengineering (LiME), Department of Mechanical Engineering, University of Victoria, 3800 Finnerty Rd., Victoria, BC, V8P 5C2, Canada; University of Victoria-Genome British Columbia Proteomics Centre, University of Victoria, 3101-4464 Markham St., Victoria, BC, V8Z 7X8, Canada.

Trends in Biotechnology
|July 31, 2017
PubMed
Summary
This summary is machine-generated.

Microfluidic chip-mass spectrometry (μchip-MS) offers a streamlined approach for early disease detection and diagnosis. This technology enhances clinical proteomics by simplifying complex sample preparation, overcoming limitations of current methods.

Keywords:
clinical translationlab-on-a-chipmass spectrometrymicrofluidicsproteomics

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Area of Science:

  • Analytical Chemistry
  • Biomedical Engineering
  • Clinical Diagnostics

Background:

  • Current clinical proteomics assays, often relying on immunoassays, face limitations in labor, cost, and complexity.
  • Mass spectrometry (MS) offers high sensitivity and specificity but requires extensive sample preparation.
  • Microfluidic chips (μchip-MS) integrate sample preparation for MS, aiming to simplify workflows.

Purpose of the Study:

  • To review recent advancements in microfluidic chip-mass spectrometry (μchip-MS) systems for clinical applications.
  • To examine the role of μchip-MS in streamlining the development and translation of MS-based proteomic assays.
  • To highlight how μchip-MS can overcome barriers to widespread clinical adoption of MS in diagnostics.

Main Methods:

  • Literature review of recent μchip-MS systems and their applications.
  • Analysis of the integration of sample preparation with MS on microfluidic platforms.
  • Evaluation of the potential of μchip-MS to address limitations of conventional clinical proteomics.

Main Results:

  • μchip-MS systems provide integrated, high-throughput sample preparation for MS.
  • These systems offer a pathway to sensitive and specific proteomic assays without traditional complexities.
  • Emerging μchip-MS platforms are poised to facilitate clinical translation of MS-based diagnostics.

Conclusions:

  • μchip-MS represents a significant advancement for clinical diagnostics and disease monitoring.
  • The technology streamlines complex proteomic workflows, making MS more accessible for clinical use.
  • μchip-MS has the potential to revolutionize early disease detection and personalized medicine through improved proteomic analysis.